LC-MS and LC-MS/MS characterization of vicriviroc metabolites
Vicriviroc and Metabolites | HPLC Retention Time | LC-MS Detected Molecular Ion | LC-MS/MS Detected Fragment Ions | Additional Structural Confirmation |
|---|---|---|---|---|
| min | m/z | |||
| Vicriviroc (SCH 417690) | 30.6 | 534 | 101, 135, 203, 232, 271, 303, 315 | Reference standard |
| M2/M3 (vicriviroc-N-oxide, SCH 643188) | 27.5/27.7 | 550 | 101, 151, 203, 248, 271, 303, 315 | Reference standard and LC-APCI-MS |
| M7 (vicriviroc-hydroxylamine, SCH 727390) | 24.8 | 538 | 101, 139, 203, 236, 271, 303, 315 | Reference standard, hydrogen-deuterium exchange and LC-APCI-MS |
| M15 (O-desmethyl-vicriviroc, SCH 495415) | 22.5 | 520 | 101, 135, 189, 232, 271, 289, 301 | Reference Standard |
| M41 (N-desalkyl-vicriviroc, SCH 496903) | 6.4 | 332 | 101, 135, 232 | Reference standard |