Kinetic parameters of DHEA-3S 16α-hydroxylation by CYP3As and CYP3A chimeras expressed in TN-5 cells
Maximal velocity (Vmax) and apparent affinity constant (Km) were determined by Lineweaver-Burk plot analysis. DHEA-3S 16α-hydroxylation was determined in three experiments over a DHEA concentration range of 2.5 to 100 μM.