Primer and probe sequences for RT-PCR
Oligonucleotide Sequences | |
|---|---|
| Conventional RT-PCR | |
| PXR sense | 5′-CAACACAGTGTTCAACGCG-3′ |
| PXR antisense | 5′-GAAGAGGGAGATGGCCTGC-3′ |
| GAPDH sense | 5′-AACAGCCTCAAGATCATCAGC-3′ |
| GAPDH antisense | 5′-GGATGATGTTCTGGAGAGCC-3′ |
| CYP3A4 sense | 5′-TGATTCCAAGCTATGCTCTTCACCG-3′ |
| CYP3A4 antisense | 5′-CTGGTTGAAGAAGTCCTCCTAAGCT-3′ |
| Real-time PCR | |
| CYP3A4 sense | 5′-GATTGACTCTCAGAATTCAAAAGAAACTGA-3′ |
| CYP3A4 antisense | 5′-GGTGAGTGGCCAGTTCATACATAATG-3′ |
| CYP3A4 probe | 5′-FAM-AGGAGAGAACACTGCTCGTGGTTTCACAG-TAMRA-3′ |
| GAPDH sense | 5′-GAAGGTGAAGGTCGGAGTC-3′ |
| GAPDH antisense | 5′-GAAGATGGTGATGGGATTTC-3′ |
| GAPDH probe | 5′-FAM-CAAGCTTCCCGTTCTCAGCC-TAMRA-3′ |