Kinetic parameters for estradiol 3-O-glucuronide formation by UGT1A1 in single and double expression systems
Data are means ± S.D. of three independent determinations.
Clone | S50 | Vmax | Vmax/S50 | n |
|---|---|---|---|---|
| μM | pmol/min/unit | μl/min/unit | ||
| 1A1 | 8.5 ± 0.2 | 983.7 ± 21.2 | 115.7 ± 1.2 | 1.8 ± 0.1 |
| 19-1 | 8.0 ± 0.3 | 523.8 ± 7.4* | 64.2 ± 3.4* | 2.1 ± 0.2 |
| 19-2 | 7.6 ± 0.5 | 439.1 ± 14.5* | 55.5 ± 4.5* | 2.1 ± 0.2 |
| 19-3 | 8.5 ± 0.7 | 174.1 ± 3.7* | 20.6 ± 1.5* | 1.9 ± 0.1 |
↵* P < 0.01 compared with the single expression system.