Kinetic parameters for serotonin O-glucuronide formation by UGT1A6 in single and double expression systems
Data are means ± S.D. of three independent determinations.
Clone | Km | Vmax | Vmax/Km |
|---|---|---|---|
| mM | nmol/min/unit | nl/min/unit | |
| 1A6 | 4.8 ± 0.2 | 1.9 ± 0.1 | 391.6 ± 4.1 |
| 69-1 | 5.2 ± 0.2* | 1.6 ± 0.1* | 309.5 ± 8.3* |
| 69-2 | 5.1 ± 0.2* | 1.6 ± 0.1* | 313.6 ± 11.8* |
| 69-3 | 5.5 ± 0.2* | 1.6 ± 0.1* | 297.0 ± 1.1* |
↵* P < 0.01 compared with the single expression system.