Primers used for mutant construction and cloning
Name | Sequence | Features |
|---|---|---|
| m-h2-1e2tF | 5′-CAGGAGGGCAGCACCCTTAATTCTCATCTCCTGCC-3′a | QEGST peptide |
| m-h2-1e2tR | 5′-GGTGCTGCCCTCCTGTGTCAAGCAGAAAATGCA-3′a | QEGST rc peptide |
| m-h2-1w4tF | 5′-AGACCCCACGCCGGCTTTCTTAATTCTCATCTCCTG-3′a | PHAG peptide |
| m-h2-1w4tR | 5′-AAAGCCGGCGTGGGGTCTCTCTTCTGTCAAGCAGA-3′a | PHAG rc peptide |
| m-h2-2bszF | 5′-GACAGAAGCCAACGACCACCTTAATTCTCATCTCCTGCC-3′a | ANDH peptide |
| m-h2-2bszR | 5′-AAGGTGGTCGTTGGCTTCTGTCAAGCAGAAAATGC-3′a | ANDH rc peptide |
| m-h2-CustF | 5′-ACAAGCGACGCCAGCGACCTTAATTCTCATCTCCTGCC-3′a | SDGSD peptide |
| m-h2-CustR | 5′-GTCGCTGGCGTCGCTTGTCAAGCAGAAAATGCA-3′a | SDGSD rc peptide |
| 3d-h2-1e2tF | 5′-ATCCACCACGAGCACCACCAAAAAATATACTTATTTACGC-3′a | HHEH peptide |
| 3d-h2-1e2tR | 5′-GTGGTGCTCGTGGTGGATTTGGTCCAGGTACCAG-3′a | HHEH rc peptide |
| 3d-h2-1w4tF | 5′-ATCGGCAGAAGCGGCCACCAAAAAATATACTTATTTACGC-3′a | GRSG peptide |
| 3d-h2-1w4tR | 5′-GTGGCCGCTTCTGCCGATTTGGTCCAGGTACCAG-3′a | GRSG rc peptide |
| 3d-h2-2bszF | 5′-ATCAGGGGCGGCAAACACCAAAAAATATACTTATTTACG-3′a | GG peptide |
| 3d-h2-2bszR | 5′-GTGTTTGCCGCCCCTGATTTGGTCCAGGTAC-3′a | GG rc peptide |
| 3d-h2-CustF | 5′-ATCGGCGGCAGCGGCCACCAAAAAATATACTTATTTACGC-3′a | CGSG peptide |
| 3d-h2-CustR | 5′-GCCGCTGCCGCCGATTTGGTCCAGGTACCA-3′a | GGSG rc peptide |
| hNat2EcoRI-F | 5′-ATGCGAGAATTCATGGACATTGAAGCATATTTTGAAAGAATT-3′b | PCR, EcoR1 |
| hNat2HindIII-R | 5′-ATGCGAAAGCTTAAGGGTTTATTTTGTTCCTTATTCTAAAT-3′b | PCR, HindIII |
| pKK-F | 5′-TTATCAGACCGCTTCTGCGT-3′ | PCR, Fwd-Seq |
| h2-Seq1F | 5′-CCGGGGTGGGTGGTGTCT-3′ | Fwd-Seq |
| h2-Seq2F | 5′-CACCTTCTCCTGCAGGTGACCAT-3′ | Fwd-Seq |
| h2-Seq3F | 5′-TTTGGTGGGCTTCATCCTCA-3′ | Fwd-Seq |
| pKK-R | 5′-GCTGTTGACAATTAATCATCGG-3′ | PCR, Rev-Seq |
| h2-Seq1R | 5′-CAACCTCTTCCTCAGTGAGAG-3′ | Rev-Seq |
| h2-Seq2R | 5′-ACATCTGGGAGGAGCTTCCA-3′ | Rev-Seq |
| h2-Seq3R | 5′-TGACCTGGAGACACCACCCA-3′ | Rev-Seq |