TABLE 2

Metabolic rates during 27 h of incubation and percentages of retained metabolic rates compared with 0 to 3 h of incubation in small intestinal slices

Results (mean ± S.E.M.) are extracted from data in Figs. 2 and 6 (n = 3-8 rats); 7HC data are extracted from not shown data.


Metabolite Formed

0-3 h (100%)

5-8 h

8-11 h

24-27 h
pmol/min/mg protein (%)
7HC (CYP1A, 1B) 18 ± 3.2 14.9 ± 5.9 (83) 16.3 ± 4.2 (91) 6.8 ± 4.5 (38)*
6β-TOH (CYP3A) 16.1 ± 3.4 7.8 ± 1.5 (48) 11.3 ± 1.2 (70) 3.9 ± 1.1(24)**
Androstenedione (several CYPs, 17β-HSD) 147 ± 22 143 ± 32 (97) 201 ± 23 (137) 93 ± 29 (63)
7HC-GLUC 377 ± 93 259 ± 38 (69) 341 ± 53 (91) 162 ± 31 (43)
7HC-SULF
39 ± 9.6
41 ± 8.1 (105)
45 ± 8.2 (115)
30 ± 8.4 (77)
  • P450, cytochrome P450; 17β-HSD, 17β-hydroxysteroid dehydrogenase.

  • * Significantly different from 0-3 h with p < 0.05.

  • ** Significantly different from 0-3 h with p < 0.01.