Effect of perinatal low-protein diets on activities of testosterone hydroxylases in 28- and 65-day-old male and female rats
Testosterone 6β-hydroxylase activity is routinely used as a marker of CYP3A1 activity in females and CYP3A1 and CYP3A2 activity in males. Testosterone 2α-hydroxylase activity is routinely used as a marker of CYP2C11 activity in males and CYP2C6 activity in females. Testosterone 7α-hydroxylase activity is routinely used as a marker of CYP2A1 activity. Values are mean (S.D.), n = 7–8. Differences between low-protein diet groups and the nonpurified diet group were not evaluated.
Male | Female | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M76 | LM76 | C93 | L93 | NP | M76 | LM76 | C93 | L93 | NP | ||||||||||
| peak area ratio/min/mg microsomal protein | |||||||||||||||||||
| Testosterone 6β hydroxylase | |||||||||||||||||||
| Day 28 | 0.159# | 0.154 | 0.211 | 0.151 | 0.254 | 0.104# | 0.128 | 0.176 | 0.167 | 0.231 | |||||||||
| (0.037) | (0.055) | (0.069) | (0.046) | (0.089) | (0.049) | (0.054) | (0.050) | (0.051) | (0.065) | ||||||||||
| Day 65 | 0.193† | 0.242† | 0.187† | 0.260† | 0.209† | 0.024 | 0.034* | 0.022 | 0.028 | 0.023 | |||||||||
| (0.050) | (0.047) | (0.028) | (0.044) | (0.051) | (0.004) | (0.007) | (0.009) | (0.004) | (0.005) | ||||||||||
| Testosterone 2α hydroxylase | |||||||||||||||||||
| Day 28 | 0.0092 | 0.0022*,† | 0.0195 | 0.0023*,† | 0.0129 | 0.0110 | 0.0047* | 0.0090 | 0.0046 | 0.0089 | |||||||||
| (0.0026) | (0.0004) | (0.0102) | (0.0006)# | (0.0070) | (0.0043) | (0.0027) | (0.0024) | (0.0009) | (0.0039) | ||||||||||
| Day 65 | 0.1088† | 0.1178† | 0.1326† | 0.1114† | 0.1136† | 0.0034 | 0.0038 | 0.0033 | 0.0033 | 0.0036 | |||||||||
| (0.0107) | (0.0343) | (0.0361) | (0.0245) | (0.0206) | (0.0006) | (0.005) | (0.0004) | (0.0009) | (0.0004) | ||||||||||
| Testosterone 7α hydroxylase | |||||||||||||||||||
| Day 28 | 0.0614 | 0.0920* | 0.0747 | 0.0847 | 0.0777† | 0.0808# | 0.1000 | 0.1007 | 0.1042 | 0.1173 | |||||||||
| (0.0278) | (0.0183) | (0.0157) | (0.0185) | (0.0108) | (0.0086) | (0.0196) | (0.0150) | (0.0189) | (0.0240) | ||||||||||
| Day 65 | 0.0274† | 0.0232† | 0.0257† | 0.0245† | 0.0265† | 0.1012 | 0.1008 | 0.1047 | 0.0954 | 0.0841 | |||||||||
|
| (0.0046) | (0.0049) | (0.0022) | (0.0075) | (0.0078) | (0.0190) | (0.0181) | (0.0371) | (0.0243) | (0.0120) | |||||||||