Primers and probes used in quantitative RT-PCR
Sequences were designed using Primer Express.
Primer or Probe | Sequence |
|---|---|
| Rat cyclophilin | |
| Forward Primer | 5′-CGATGACGAGCCCTTGG-3′ |
| Reverse Primer | 5′-TCTGCTGTCTTTGGAACTTTGTC-3′ |
| Probe | 6FAM-CGCGTCTGCTTCGAGCTGTTTGCA-BHQ |
| Rat CYP4A1 | |
| Forward Primer | 5′-TCACCTCCCTTCCACTGGTT-3′ |
| Reverse Primer | 5′-TCCACACATGTCATAATTTGCT-3′ |
| Probe | 6FAM-TCACCTTGAAACTGCTTGTGCCCA-BHQ |
| Rat sEH | |
| Forward Primer | 5′-CTCTAAACTGGTATCGAAACACAGAAAG-3′ |
| Reverse Primer | 5′-ATGTCCTTCTCAGCTGTGACCAT-3′ |
| Probe | 6FAM-CGTTGGGAAGGAAGATCTTGGTCCCT-BHQ |
| Rat CYP2C11 | |
| Forward Primer | 5′-GCCTTGTGGAGGAACTGAGG-3′ |
| Reverse Primer | 5′-AGCACAGCCCAGGATAAAGGT-3′ |
| Probe | 6FAM-AGCAAAGGTGCCCCTTTTGATCCC-TAMRA |
| Rat CYP2C23 | |
| Forward Primer | 5′-TTCGGGCTCCTGCTCCTTA-3′ |
| Reverse Primer | 5′-CGTCCAATCACACGGTCAAG-5′ |
| Probe | 6FAM-AGAGGTGCAAGCCAAAGTTCATGAGGA-BHQ |