LC-MS comparison of temsirolimus metabolic products from human liver microsomes and from recombinant CYP3A4
Products from HLM (Retention Time)a | [M-H]−m/zb | Products from CYP3A4 (Retention Time)a | [M-H]−m/zb |
|---|---|---|---|
| Peak 1-2 (29.8-31.2 min) | 1060, 1058 | Peak 1-2 (31.0-31.9 min) | 1060, 1046 |
| Peak 3-4 (35.7-36.2 min) | 1060, 1046, 1014 | Peak 3 (32.4 min) | 1030 |
| Peak 5 (3638.2 min) | 1030 | Peak 4 (35.7 min) | 1014 |
| Peak 6 (40.1 min) | 1026 | Peak 5-6 (38.3-39.8 min) | 1026, 1028 |
| Seco-temsirolimus (43.9 min) | 1028 | Seco-temsirolimus (44.1 min) | 1028 |
| Peak 7 (47.0 min) | 1030 | Peak 7 (47.6 min) | 1030 |
| M8 (54.5 min) | 1044 | M8 (54.6 min) | 1044 |
| M9 (58.0 min) | 1044 | M9 (58.3 min) | 1044 |
| M10 (61.2 min) | 1044 | M10 (61.8 min) | 1044 |
| M11 (64.4 min) | 1044 | M11 (64.9 min) | 1044 |
| Peak 11′ (66.2 min) | 1030 | ||
| M12 (69.8 min) | 1044 | M12 (69.8 min) | 1044 |
| M13 (83.3 min) | 1014 | M13 (83.1 min) | 1014 |
| M14 (87.4 min) | 1028 | ||
| Temsirolimusc (87.9-90 min) | 1028 | Temsirolimus (88.2-90 min) | 1028 |