Summary of names, sequences and utilities of oligonucleotides used in this study
Oligonucleotide | Sequence | Utility |
|---|---|---|
| NATbqF | 5′-CTGGTTGCCGGCTGAAATAAC-3′ | NATb q-RT-PCR Forward |
| NATa/bqR | 5′-TCCAAGTCCAATTTGTTCCTAGACT-3′ | NAT1 q-RT-PCR Reverse |
| NATa/bpr | 6FAM5′-CAATCTGTCTTCTGGATTAA-3′MGBNFQ | NAT1 TaqMan Probe |
| NATb 318H3 | 5′-CCCCCAAGCTTAAGAGCACAGTCCTCCCAGC-3′ | 318 Forward |
| NATb 245H3 | 5′-CCCCCAAGCTTGATATGAATAAGGGGTCCACAG-3′ | 245 Forward |
| NATb 213H3 | 5′-CCCCCAAGCTTAGTCCCTGCAACTACATTTCC-3′ | 213 Forward |
| NATb 178H3 | 5′-CCCCCAAGCTTGTAACCCTAATGTGACTCTGGG-3′ | 178 Forward |
| NATb 150H3 | 5′-CCCCCAAGCTTGCCAGTTGGACATCCAATG-3′ | 150 Forward |
| NAT1TSSZeroRevH3 | 5′-CCCCCCAAGCTTAGCCCCTTACAGAGTTCCACTTAC-3′ | Promoter clone Reverse |
| Con1-L9BamFor | 5′-CCCCGGATCCAGCCATTTCCCAGGGGC-3′ | Mutant Con1-L9 |
| Con1-L9BamRev | 5′-CCCCGGATCCGGACTCAGGTTGCTCCT-3′ | Mutant Con1-L9 |
| Con1-R7BamFor | 5′-CCCCGGATCCGGGCCCCTGGGGTA-3′ | Mutant Con1-R7 |
| Con1-R7BamRev | 5′-CCCCGGATCCCATGTAGTTGCAGGGACTCAG-3′ | Mutant Con1-R7 |
| Pal 11/16ApoFor | 5′-GGATCCAAATTTGTAACCCTAATGTGACTCTGGG-3′ | Mutant Pal 11/16 |
| Pal 11/16ApoRev | 5′-GGATCCAAATTTTTAAACTGGGAAATGTAGTTGCAGG-3′ | Mutant Pal 11/16 |
| AP1-5EcoFor | 5′-CCCCCCGAATTCCTGGGGGCGGGGCCAGT-3′ | Mutant AP1-5 |
| AP1-5EcoRev | 5′-CCCCCCGAATTCATTAGGGTTACCCCAGG-3′ | Mutant AP1-5 |
| Sp1del4, GC>ATBamFor | 5′-CCCCGGATCCAGTTGGACATCCAATGA-3′ | Mutant Sp1del4, GC >AT |
| Sp1del4, GC>ATBamRev | 5′-CCCCGGATCCCCAGAGTCACATTAGGGT-3′ | Mutant Sp1del4, GC >AT |
| Sp1G3G4>ATFor | 5′-GACTCTGGGATCGGGGCCAG-3′ | Mutant Sp1G3G4 >AT |
| Sp1G3G4>ATRev | 5′-CTGGCCCCGATCCCAGAGTC-3′ | Mutant Sp1G3G4 >AT |
| NF-Y-6EcoFor | 5′-CCCCCCGAATTCTGAGAGCACTTCCCCTG-3′ | Mutant NF-Y-6 |
| NF-Y-6EcoRev | 5′-CCCCCCGAATTCGTCCAACTGGCCCCG -3′ | Mutant NF-Y-6 |
| Dup1-R7PstFor | 5′-CTTCCCCTGCAGACTTTGGA-3′ | Mutant Dup1-R7 |
| Dup1-R7PstRev | 5′-CCCCCCCTGCAGGTTCCTGTCTCTCATTGGATGTCCAACT-3′ | Mutant Dup1-R7 |
| Dup2-L6For | 5′-GGAAGGTCTCTCACTTCCTCATAGACC-3′ | Mutant Dup2-L6 |
| Dup2-L6Rev | 5′-AAGTGAGAGACCTTCCAAAGTCTGCA-3′ | Mutant Dup2-L6 |
| NATb Genomic-For | 5′-TCTAGCCTGCCTGAGGACAT-3′ | BAC Outer nest For |
| NATb Genomic-Rev | 5′-CTTTGGGACACACACCACTG-3′ | BAC Outer nest Rev |
| RV-3 (pGL3 Basic) | 5′-CTAGCAAAATAGGCTGTCCC-3′ | Plasmid Outer nest For |
| GL-2 (pGL3 Basic) | 5′-CTTTATGTTTTTGGCGTCTTCC-3′ | Plasmid Outer nest Rev |
| 200L29 sense | 5′-ACATTTCCCAGGGGCCCCTGGGGTAACCC-3′ | EMSA |
| 200L29 antisense | 5′-GGGTTACCCCAGGGGCCCCTGGGAAATGT-3′ | EMSA |
| 165L26 sense | 5′-GACTCTGGGGGCGGGGCCAGTTGGAC-3′ | EMSA |
| 165L26 antisense | 5′-GTCCAACTGGCCCCGCCCCCAGAGTC-3′ | EMSA |
| Sp1 consensus (sense) | 5′-ATTCGATCGGGGCGGGGCGAGC-3′ | EMSA control/competitor |
| Sp1 consensus (antisense) | 5′-GCTCGCCCCGCCCCGATCGAAT-3′ | EMSA control/competitor |
| AP-1 consensus (sense) | 5′-CGCTTGATGAGTCAGCCGGAA-3′ | EMSA competitor |
| AP-1 consensus (antisense) | 5′-TTCCGGCTGACTCATCAAGCG-3′ | EMSA competitor |
| AP-2 consensus (sense) | 5′-GATCGAACTGACCGCCCGCGGCCCGT-3′ | EMSA competitor |
| AP-2 consensus (antisense) | 5′-ACGGGCCGCGGGCGGTCAGTTCGATC-3′ | EMSA competitor |