Specific activities of P450 isoform-catalyzed reactions in microsomes from 10 human livers and their correlation with N-demethyldiuron formation
Each value is an average of two parallel incubations.
Human Liver | CYP1A2 | CYP2A6 | CYP2B6 | CYP2C8 | CYP2C9 | CYP2C19 | CYP2D6 | CYP2E1 | CYP3A4a | CYP3A4b | N-Demethyldiuron | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| pmol min−1 mg−1 microsomal protein | nmol/mg protein/min | |||||||||||||
| HL20 | 85.9 | 95.8 | 54.2 | 268.4 | 139.9 | 42.4 | 229.6 | 386.4 | 1174.9 | 17.2 | 1.33 | |||
| HL21 | 31.5 | 465.5 | 77.2 | 219.2 | 128.4 | 21.7 | 215.3 | 686.7 | 772.9 | 30.2 | 0.66 | |||
| HL22 | 31.1 | 323.8 | 118.2 | 221.9 | 103.2 | 105.7 | 194.2 | 314.4 | 3142.7 | 114.3 | 0.62 | |||
| HL23 | 59.5 | 123.1 | 42.6 | 229.6 | 148.2 | 36.3 | 213.0 | 269.3 | 611.3 | 38.2 | 1.50 | |||
| HL24 | 57.3 | 110.6 | 102.6 | 215.9 | 79.8 | 58.3 | 107.2 | 254.2 | 1034.4 | 30.2 | 1.26 | |||
| HL28 | 109.8 | 549.2 | 4.5 | 332.7 | 139.3 | 124.0 | 154.1 | 773.5 | 4568.2 | 130.5 | 1.57 | |||
| HL29 | 40.6 | 464.5 | 220.5 | 362.4 | 269.3 | 46.7 | 187.5 | 438.2 | 1760.6 | 43.7 | 1.45 | |||
| HL30 | 61.7 | 147.0 | 40.9 | 209.3 | 98.2 | 45.4 | 67.9 | 244.7 | 813.7 | 57.7 | 0.76 | |||
| HL31 | 3.8 | 225.3 | 35.5 | 152.6 | 178.9 | 88.9 | 471.2 | 511.6 | 12.0 | 0.08 | ||||
| HL32 | 18.9 | 311.7 | 35.1 | 175.6 | 201.0 | 21.4 | 111.2 | 136.0 | 602.5 | 20.5 | 1.24 | |||
| Mean: | 50.0 | 281.6 | 73.1 | 238.7 | 148.6 | 55.8 | 156.9 | 397.4 | 1499.3 | 49.5 | 1.05 | |||
| Correlation coefficient (r) | 0.67 | 0.08 | 0.10 | 0.67 | 0.20 | 0.07 | 0.35 | −0.04 | 0.28 | 0.16 | ||||
| HKUF |
|
|
|
|
|
|
|
|
|
| 1.50 | |||