Kinetic parameters for propranolol metabolite formation
Kinetic constants were determined over the concentration ranges 1 to 100 μM (CYP2D6) or 50 to 2000 μM (CYP2C9 preparations).
Enzyme | 4-Hydroxy-propranolol | Desisopropyl-propranolol | ||||
|---|---|---|---|---|---|---|
| KM | Vm | KM | Vm | |||
| μM | min-1 | μM | min-1 | |||
| CYP2D6 | 22 ± 7 | 66 ± 11 | 14 ± 6 | 5 ± 0.07 | ||
| CYP2C9 | >900 | <0.5 | >150 | <0.5 | ||
| R108E | >900 | <0.5 | >150 | <0.5 | ||
| R108E/D293N | >900 | <0.5 | >150 | <0.5 | ||