TABLE 2

Effect of polymeric support on the production of 32-hydroxy-rifalazil catalyzed by MLM

Reaction conditions: 100 mM potassium phosphate buffer, pH 7.4, containing 20 μl/ml MLM, 100 mg/l rifalazil, 5 U/ml glucose dehydrogenase, 0.25 mM NADP, 111 mM glucose. Total reaction volume was 1 ml. Reaction temperature 37°C, shaking rate 150 rpm, reaction time 16 h. Polymeric resins were added to the reaction mixture separately from rifalazil. Under these experimental conditions and using an MLM batch specific to this experiment, the volumetric productivity of the resin-free system was 0.2 mg product/l reaction volume.