TABLE 2

Correlation between Km, Embedded Image, and Embedded Image for apical efflux at various CLd when CLint,met = 0.01 ml/min and Vmax = 0.5 nmol/min CLinflux = CLefflux = CLabs = 0 and CLd1 = CLd2 = CLd3 = CLd4 = CLd; all the unbound fractions were set to unity.

Assigned Km
A → B
B → A
Jmax *
Embedded Image
Embedded Image
 β
Jmax *
Embedded Image
Embedded Image
 β
nmol/min μM μM nmol/min μM μM
Case 1 (CLd = 0.01 ml/min)
   0.05 μM 0.134 0.252 12.0 2.10 0.288 0.226 11.2 2.12
   0.5 μM 0.134 2.49 17.4 1.62 0.288 2.23 16.2 1.63
   5 μM 0.135 23.9 39.0 1.20 0.290 21.5 36.0 1.21
   25 μM 0.138 109 116 1.04 0.296 99.3 107 1.05
   50 μM 0.139 205 209 1.02 0.298 188 192 1.02
Case 2 (CLd = 0.1 ml/min)
   0.05 μM 0.0640 0.173 1.49 1.49 0.0825 0.0874 0.857 1.46
   0.5 μM 0.0640 1.60 3.57 1.21 0.0825 0.967 2.19 1.20
   5 μM 0.0640 15.8 17.7 1.04 0.0825 9.78 11.2 1.05
   25 μM 0.0640 78.6 79.4 1.01 0.0826 48.8 49.6 1.01
   50 μM 0.0640 156 156 1.00 0.0826 97.4 97.9 1.00
Case 3 (CLd = 1 ml/min)
   0.05 μM 0.00671 0.160 0.233 1.06 0.00848 0.0908 0.123 1.04
   0.5 μM 0.00671 1.58 1.78 1.03 0.00848 0.930 1.03 1.02
   5 μM 0.00671 15.8 16.0 1.01 0.00848 9.22 9.40 1.01
   25 μM 0.00672 79.4 79.3 1.00 0.00849 46.4 46.5 1.00
   50 μM
0.00671
158
158
1.00
0.00849
92.8
92.8
1.00

  • ↵* True Vmax = 0.5 nmol/min