TABLE 4

Corelation between Km (0.5 μM), Embedded Image, and Embedded Image for apical influx at various CLd when CLint,met = 0.01 ml/min and Vmax = 0.5 nmol/min CLinflux = CLefflux = CLint,sec = 0 and CLd1 = CLd2 = CLd3 = CLd4 = CLd; all the unbound fractions are set to unity.

Assigned Km
A → B
B → A
Jmax *
Embedded Image
Embedded Image
 β
Jmax *
Embedded Image
Embedded Image
 β
nmol/min μM μM nmol/min μM μM
Case 1 (CLd = 0.01 ml/min)
   0.05 μM 0.134 0.0613 3.96 2.10 0.288 4.70 31.6 1.60
   0.5 μM 0.134 0.612 5.28 1.62 0.289 25.3 51.1 1.29
   5 μM 0.134 6.11 10.3 1.18 0.284 133 140 1.04
   25 μM 0.134 30.4 33.0 1.04 0.264 450 450 1.00
   50 μM 0.134 60.3 62.1 1.02 0.253 799 799 1.00
Case 2 (CLd = 0.1 ml/min)
   0.05 μM 0.0640 0.100 3.04 1.85 0.0825 0.180 1.78 1.55
   0.5 μM 0.0640 1.34 5.22 1.39 0.0825 1.35 3.50 1.26
   5 μM 0.0641 13.7 17.4 1.09 0.0824 12.0 13.8 1.05
   25 μM 0.0642 67.4 69.2 1.02 0.0822 56.6 57.2 1.01
   50 μM 0.0643 133 134 1.00 0.0820 110 111 1.00
Case 3 (CLd = 1 ml/min)
   0.05 μM 0.00671 0.161 0.378 1.15 0.00848 0.0919 0.172 1.10
   0.5 μM 0.00671 1.58 2.02 1.06 0.00848 0.934 1.14 1.04
   5 μM 0.00671 15.8 16.3 1.01 0.00848 9.32 9.57 1.01
   25 μM 0.00671 79.1 79.4 1.00 0.00849 46.5 46.6 1.00
   50 μM
0.00672
158
158
1.00
0.00848
92.8
92.9
1.00

  • ↵* True Vmax = 0.5 nmol/min