TABLE 5

Correlation between Vmax and Jmax for cellular metabolism (Km = 0.5 μM) at various CLd when other satiable components (apical/basolateral influx, efflux) were absent CLinflux = CLefflux = CLint,sec = CLabs = 0 and CLd1 = CLd2 = CLd3 = CLd4 = CLd; all the unbound fractions were set to unity.

Assigned Vmax
A → B
B → A
Jmax
K[prime]m*
Embedded Image *
β
Jmax
Embedded Image *
Embedded Image *
β
nmol/min μM μM nmol/min μM μM
Case 1 (CLd = 0.01 ml/min)
   0.05 nmol/min 0.0256 1.13 3.40 1.29 0.0238 1.05 3.13 1.28
   0.5 nmol/min 0.256 1.75 18.4 1.72 0.238 1.57 17.1 1.73
   5 nmol/min 124 2.92 × 104 2.91 × 104 1.00 92.4 2.17 × 104 2.16 × 104 1.00
   25 nmol/min 2.81 × 104 6.82 × 106 6.82 × 106 1.00 2.65 × 104 6.27 × 106 6.27 × 106 1.00
   50 nmol/min 1.32 × 105 3.11 × 107 3.11 × 107 1.00 1.22 × 105 2.89 × 107 2.89 × 107 1.00
Case 2 (CLd = 0.1 ml/min)
   0.05 nmol/min 0.0293 1.23 1.87 1.10 0.0205 0.858 1.26 1.08
   0.5 nmol/min 0.293 1.37 8.20 1.53 0.205 0.927 5.59 1.51
   5 nmol/min 3.21 50.1 96.3 1.26 2.06 2.79 35.6 1.69
   25 nmol/min 199 1.32 × 104 1.31 × 104 1.00 267 1.77 × 104 1.76 × 104 1.00
   50 nmol/min 1.09 × 104 7.27 × 105 7.27 × 105 1.00 2.20 × 104 1.48 × 106 1.48 × 106 1.00
Case 3 (CLd = 1 ml/min)
   0.05 nmol/min 0.0310 1.34 1.80 1.07 0.0189 0.812 1.05 1.05
   0.5 nmol/min 0.310 1.43 7.63 1.51 0.189 0.846 4.52 1.47
   5 nmol/min 3.15 10.0 57.8 1.52 1.89 1.51 29.2 1.79
   25 nmol/min 1.34 × 107 8.60 × 108 8.60 × 108 1.00 1.08 × 105 6.93 × 106 6.93 × 106 1.00
   50 nmol/min
3.41 × 109
2.19 × 1011
2.19 × 1011
1.00
4.11 × 109
2.64 × 1011
2.64 × 1011
1.00

  • ↵* True Km is 0.5 μM