TABLE 6

Correlation between Km, Embedded Image, and Embedded Image for cellular metabolism at various CLd when other satiable components (apical/basolateral influx, efflux) were absent CLinflux = CLefflux = CLint,sec = CLabs = 0 and CLd1 = CLd2 = CLd3 = CLd4 = CLd; all the unbound fractions were set to unity.

Assigned Km
A → B
B → A
Jmax *
Embedded Image
Embedded Image
 β
Jmax *
Embedded Image
Embedded Image
 β
nmol/min μM μM nmol/min μM μM
Case 1 (CLd = 0.01 ml/min)
   0.05 μM 0.256 0.179 13.4 2.19 0.238 0.160 12.6 2.20
   0.5 μM 0.256 1.75 18.4 1.72 0.238 1.57 17.1 1.73
   5 μM 0.258 16.7 35.2 1.29 0.240 15.0 32.6 1.29
   25 μM 0.263 75.9 87.7 1.08 0.244 69.2 80.8 1.08
   50 μM 0.266 142 149 1.04 0.247 130 137 1.04
Case 2 (CLd = 0.1 ml/min)
   0.05 μM 0.293 0.139 6.02 2.03 0.205 0.0951 3.93 1.98
   0.5 μM 0.293 1.37 8.20 1.53 0.205 0.927 5.59 1.51
   5 μM 0.293 13.6 20.0 1.14 0.206 9.21 13.9 1.15
   25 μM 0.295 66.7 70.0 1.03 0.206 45.6 48.6 1.03
   50 μM 0.295 131 133 1.01 0.207 90.3 92.1 1.01
Case 3 (CLd = 1 ml/min)
   0.05 μM 0.310 0.143 5.40 2.00 0.189 0.0847 3.34 1.94
   0.5 μM 0.310 1.43 7.63 1.51 0.189 0.846 4.52 1.47
   5 μM 0.310 14.3 19.1 1.11 0.189 8.45 11.6 1.11
   25 μM 0.311 70.4 72.7 1.02 0.189 42.0 43.9 1.02
   50 μM
0.312
139
140
1.01
0.190
83.6
84.8
1.01

  • ↵* True Vmax = 0.5 nmol/min