Rates of O-deethylation and 3-hydroxylation of 7-ethoxycoumarin by various CYP102A1 mutants
Assays were performed using 2.0 mM 7-ethoxycoumarin as described under Materials and Methods. Values are the mean ± S.D. of triplicate determinations.
Enzyme | O-Deethylation | 3-Hydroxylation | |
|---|---|---|---|
| nmol product/min/nmol P450 | |||
| Wild type | 0.11 ± 0.01 | 0.14 ± 0.01 | |
| Mutant | |||
| 1 | 0.78 ± 0.01 | 1.7 ± 0.1 | |
| 2 | 0.89 ± 0.01 | 0.86 ± 0.02 | |
| 3 | 0.98 ± 0.04 | 1.1 ± 0.1 | |
| 4 | 0.22 ± 0.01 | 0.35 ± 0.01 | |
| 5 | 2.7 ± 0.1 | 2.5 ± 0.1 | |
| 6 | 0.44 ± 0.02 | 1.3 ± 0.1 | |
| 7 | 1.1 ± 0.1 | 1.6 ± 0.1 | |
| 8 | 11 ± 1 | 18 ± 1 | |
| 9 | 6.7 ± 0.1 | 12 ± 1 | |
| 10 | 5.8 ± 0.4 | 17 ± 1 | |
| 11 | 0.65 ± 0.01 | 2.3 ± 0.1 | |
| 12 | 0.68 ± 0.01 | 1.6 ± 0.1 | |
| 13 | 3.1 ± 0.1 | 13 ± 1 | |
| 14 | 2.3 ± 0.1 | 7.7 ± 0.1 | |
| 15 | 2.6 ± 0.1 | 8.6 ± 0.1 | |
| 16 | 2.8 ± 0.1 | 15 ± 1 | |
| 17 | 2.9 ± 0.2 | 16 ± 1 | |