Enzyme | O-Deethylation | 3-Hydroxylation | |
---|---|---|---|
nmol product/min/nmol P450 | |||
Wild type | 0.11 ± 0.01 | 0.14 ± 0.01 | |
Mutant | |||
1 | 0.78 ± 0.01 | 1.7 ± 0.1 | |
2 | 0.89 ± 0.01 | 0.86 ± 0.02 | |
3 | 0.98 ± 0.04 | 1.1 ± 0.1 | |
4 | 0.22 ± 0.01 | 0.35 ± 0.01 | |
5 | 2.7 ± 0.1 | 2.5 ± 0.1 | |
6 | 0.44 ± 0.02 | 1.3 ± 0.1 | |
7 | 1.1 ± 0.1 | 1.6 ± 0.1 | |
8 | 11 ± 1 | 18 ± 1 | |
9 | 6.7 ± 0.1 | 12 ± 1 | |
10 | 5.8 ± 0.4 | 17 ± 1 | |
11 | 0.65 ± 0.01 | 2.3 ± 0.1 | |
12 | 0.68 ± 0.01 | 1.6 ± 0.1 | |
13 | 3.1 ± 0.1 | 13 ± 1 | |
14 | 2.3 ± 0.1 | 7.7 ± 0.1 | |
15 | 2.6 ± 0.1 | 8.6 ± 0.1 | |
16 | 2.8 ± 0.1 | 15 ± 1 | |
17 | 2.9 ± 0.2 | 16 ± 1 |