P450 | 7-Ethoxycoumarin | O-Deethylation | 3-Hydroxylation | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
kcat | Km | kcat/Km | DV | D(V/K) | kcat | Km | kcat/Km | DV | D(V/K) | ||||||||||
min−1 | μM | min−1 | μM | ||||||||||||||||
Mutant 8 | d 0 | 17 ± 1 | 1050 ± 140 | 0.016 ± 0.002 | 11 ± 1 | 11 ± 2 | 25 ± 2 | 1050 ± 150 | 0.024 ± 0.004 | 0.96 ± 0.11 | 1.0 ± 0.2 | ||||||||
d 2 | 1.6 ± 0.1 | 1130 ± 160 | 0.0014 ± 0.0002 | 26 ± 2 | 1120 ± 150 | 0.023 ± 0.004 | |||||||||||||
Mutant 10 | d 0 | 10 ± 1 | 1220 ± 240 | 0.010 ± 0.002 | 10 ± 1 | 14 ± 4 | 29.5 ± 3.4 | 1340 ± 290 | 0.022 ± 0.005 | 0.90 ± 0.14 | 0.96 ± 0.30 | ||||||||
d 2 | 1.0 ± 0.1 | 1360 ± 230 | 0.00074 ± 0.00011 | 32.6 ± 3.2 | 1400 ± 260 | 0.023 ± 0.005 | |||||||||||||
Mutant 11 | d 0 | 1.7 ± 0.1 | 45 ± 5 | 0.038 ± 0.005 | 8.1 ± 0.6 | 13 ± 2 | 6.4 ± 0.2 | 81 ± 10 | 0.079 ± 0.010 | 0.79 ± 0.03 | 1.1 ± 0.2 | ||||||||
d 2 | 0.21 ± 0.01 | 72 ± 8 | 0.0029 ± 0.0004 | 8.1 ± 0.2 | 118 ± 10 | 0.069 ± 0.006 | |||||||||||||
Mutant 12 | d 0 | 0.68 ± 0.01 | 48 ± 3 | 0.014 ± 0.001 | 6.2 ± 0.6 | 17 ± 3 | 2.0 ± 0.1 | 427 ± 29 | 0.0047 ± 0.0004 | 0.71 ± 0.04 | 1.1 ± 0.1 | ||||||||
d 2 | 0.11 ± 0.01 | 131 ± 15 | 0.00084 ± 0.00012 | 2.8 ± 0.1 | 682 ± 45 | 0.0041 ± 0.0003 | |||||||||||||
Mutant 17 | d 0 | 2.9 ± 0.1 | 160 ± 12 | 0.0018 ± 0.001 | 9.4 ± 0.4 | 12 ± 1 | 16.5 ± 0.4 | 225 ± 23 | 0.073 ± 0.008 | 0.94 ± 0.04 | 0.99 ± 0.11 | ||||||||
|
d
2
| 0.31 ± 0.01 | 203 ± 15 | 0.0015 ± 0.0001 |
|
| 17.6 ± 0.7 | 237 ± 35 | 0.074 ± 0.002 |
|
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