TABLE 2

Steady-state kinetics for 7-hydroxywarfarin glucuronidation by UGT1A10 and its mutants

Enzyme	Kinetic Model	Kinetic Parameters
Enzyme	Kinetic Model			V_max	K_m	K_s
		nmol/min/mg protein	μM	μM
Wild type	Michaelis-Menten	1.1 (1.0-1.3)^a	170 (77-250)^a	—^b
F90A	—^b	—^b	—^b	—^b
V92A	Substrate inhibition	2.9 (1.9-7.5)^a	560 (270-1900)^a	1100 (320-2800)^a
F93A	Substrate inhibition	N.D.	N.D.	N.D.

N.D., not able to be determined (see Results).
↵ a 95% confidance intervals.
↵ b No activity was detected.