Steady-state kinetics for 8-hydroxywarfarin glucuronidation by UGT1A10 and its mutants
Enzyme | Kinetic model | Kinetic Parameters | ||
|---|---|---|---|---|
| Vmax | Km | |||
| mnol/min/mg protein | μM | |||
| Wild type | Michaelis-Menten | 25 (22-28)a | 390 (240-530)a | |
| F90A | Michaelis-Menten | 18 (15-20)a | 190 (73-310) | |
| V92A | Michaelis-Menten | 260 (240-280)a | 390 (300-480) | |
| F93A | Michaelis-Menten | 69 (63-75)a | 304 (220-400) | |
↵ a 95% confidence intervals.