Values | Phosphodiesterase I (0.3 U/ml)a | HLM (0.2 mg protein/ml)a | HLM (0.2 mg protein/ml)b |
---|---|---|---|
nmol/h | nmol/h/mg | nmol/h/mg | |
V max | 0.73 ± 0.06b | 16.8 ± 8.0 | 16.5 ± 8.4 |
Km (μM) | 1.28 ± 0.42 | 12.1 ± 8.6 | 6.33 ± 3.2 |
n
c
| 0.97 ± 0.05 | 1.28 ± 0.4 | 0.814 ± 0.09 |
↵ a Results are presented as formation rates of sum of 3′N-1 and 3′N-2 vs. total substrate concentrations. They were all determined in triplicate and from triplicate experiments.
↵ b Results are presented as formation rates of sum of 3′N-1 and 3′N-2 vs. unbound substrate concentrations, all from triplicate experiments.
↵ c Hill coefficient.