Platinum accumulation in colorectal cancer-derived cell lines (2 min)
Colorectal cancer-derived cell lines were treated with medium containing 100, 500, or 1000 μM oxaliplatin for 2 min. After being washed, these cells were solubilized in 0.5 N NaOH, and the amount of platinum was determined by ICP-MS. Each value represents the mean ± S.E. for four wells.
Cell Lines | Platinum Accumulation | ||||
|---|---|---|---|---|---|
| 100 μM | 500 μM | 1000 μM | |||
| ng/mg protein/2 min | |||||
| T84 | 0.82 ± 0.03 | 5.12 ± 0.06 | 9.77 ± 0.46 | ||
| SW480 | 0.48 ± 0.01 | 2.27 ± 0.02 | 4.19 ± 0.04 | ||
| HCT116 | 0.46 ± 0.03 | 2.72 ± 0.08 | 5.23 ± 0.26 | ||
| HT29 | 0.64 ± 0.01 | 3.55 ± 0.05 | 7.85 ± 0.19 | ||
| SW837 | 1.07 ± 0.02 | 5.77 ± 0.10 | 14.3 ± 1.13 | ||
| Lovo | 0.61 ± 0.01 | 3.59 ± 0.07 | 7.27 ± 0.10 | ||