Blood pH and electrolyte concentrations before and after GHB administration alone or with concomitant l-lactate and/or d-mannitol treatments
GHB (208 mg/h/kg) was administered by i.v. infusion to reach steady-state concentration. l-Lactate (60.5, 121, and 302 mg/h/kg) was administered by i.v. infusion for 3 h. d-Mannitol (0.5g/kg) was administered by i.v. bolus. For the combined treatment, l-lactate (60.5 mg/h/kg) was administered by i.v. infusion for 3 h, and d-mannitol (0.5g/kg) was administered by i.v. bolus. Blood pH and electrolyte concentrations before and after the infusion were measured using an ABL 605 Radiometer. The results are expressed as mean ± S.D., n = 3 to 5. A paired Student's t test was used to detect statistical significance.
GHB Only | l-Lactate (60.5 mg/h/kg) | l-Lactate (121 mg/h/kg) | l-Lactate (302.5 mg/h/kg) | d-Mannitol (0.5 g/kg) | d-Mannitol + l-Lactate | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 min | 180 min | 0 min | 180 min | 0 min | 180 min | 0 min | 180 min | 0 min | 180 min | 0 min | 180 min | |||||||
| pH | 7.44 ± 0.02 | 7.48 ± 0.02 | 7.43 ± 0.01 | 7.52 ± 0.01** | 7.46 ± 0.01 | 7.52 ± 0.02* | 7.46 ± 0.01 | 7.53 ± 0.01*** | 7.46 ± 0.01 | 7.48 ± 0.03* | 7.44 ± 0.01 | 7.49 ± 0.01*** | ||||||
| K+ (mM) | 4.1 ± 0.5 | 3.7 ± 0.4 | 4.2 ± 0.4 | 4.0 ± 0.4 | 4.5 ± 0.7 | 4.0 ± 0.7 | 3.8 ± 0.2 | 3.5 ± 0.2 | 4.0 ± 0.2 | 3.4 ± 0.2* | 4.2 ± 0.5 | 3.6 ± 0.3* | ||||||
| Na+ (mM) | 145 ± 2 | 147 ± 2 | 145 ± 2 | 145 ± 2 | 145 ± 2 | 145 ± 2 | 145 ± 2 | 146 ± 3 | 141 ± 1 | 143 ± 1 | 142 ± 1 | 143 ± 2 | ||||||
| Cl− (mM) | 108 ± 2 | 99 ± 2** | 107 ± 2 | 97 ± 2** | 107 ± 3 | 97 ± 2* | 108 ± 4 | 95 ± 1** | 104 ± 2 | 100 ± 1 | 106 ± 4 | 96 ± 3*** | ||||||
| Ca2+ (mM) | 0.98 ± 0.20 | 0.96 ± 0.12 | 0.90 ± 0.17 | 0.77 ± 0.20 | 0.79 ± 0.14 | 0.80 ± 0.04 | 0.82 ± 0.09 | 0.96 ± 0.31 | 1.22 ± 0.06 | 1.17 ± 0.04 | 1.25 ± 0.09 | 1.16 ± 0.17 | ||||||
| Anion gap (mM) | 14.9 ± 2.4 | 19.8 ± 3.0* | 17.5 ± 2.2 | 19.5 ± 2.0 | 16.7 ± 2.1 | 18.3 ± 1.8 | 14.2 ± 2.7 | 17.1 ± 3.7* | 13.3 ± 0.8 | 16.7 ± 0.3 | 12.7 ± 0.9 | 16.2 ± 2.4* | ||||||
| Glucose (mM) | 7.5 ± 0.6 | 9.5 ± 1.6* | 8.1 ± 0.3 | 7.3 ± 1.2 | 7.0 ± 0.2 | 9.3 ± 0.8* | 6.5 ± 0.7 | 8.9 ± 0.7** | 8.4 ± 1.8 | 10.0 ± 1.2 | 6.7 ± 0.8 | 9.5 ± 2.0* | ||||||
| l-Lactate (mM) | 1.1 ± 0.2 | 1.1 ± 0.2 | 1.1 ± 0.2 | 1.4 ± 0.5 | 1.0 ± 0.1 | 1.4 ± 0.1** | 0.8 ± 0.1 | 1.4 ± 0.2** | 1.2 ± 0.4 | 0.9 ± 0.1 | 1.0 ± 0.3 | 1.3 ± 0.4 | ||||||