TABLE 1

The expression of different UGT enzymes in Caco-2 cells at several passage levels determined by qRT-PCR

Expression levels relative to β-actin: +, >0.0005; ++, >0.0015; +++, > 0.005; ++++, > 0.01.

Enzyme
P31
P37
P43
P49
D
U
D
U
D
U
D
U
UGT1A1 + ± + ± ++ ± + ±
UGT1A4 + ± + ± + ± ± ±
UGT1A5 + ± + ± ++ ± + +
UGT1A6 ++++ +++ ++++ +++ ++++ ++++ +++ +++
UGT1A7 ++ ± + ± ++ + + +
UGT1A8 ++ ± ++ ± +++ ± ++ +
UGT1A9 + ± + ± ++ ± + +
UGT1A10 + ± + ± ++ ± + ±
UGT2B4 ± ± ± ± ± ± + ±
UGT2B7 ++ ± + ± ++ ± + ±
UGT2B10 ± ± ± ± ± ± ± ±
UGT2B11 ++ ± + ± ++ + ++ ++
UGT2B15 + ± ± ± ± ± ± ±
UGT2B17 ++ ± ++ ± +++ ± +++ ±
UGT2B28
+
±
±
±
±
±
±
±

  • D, fully differentiated cell samples grown on filters for at least 21 days; U, undifferentiated samples obtained from culturing flasks.