Nucleotide sequences of PCR primers
Gene | Primer Sequence (5′-3′) | Predicted Product Size |
|---|---|---|
| base pair | ||
| mdr1a | Sens: ATCAACTCGCAAAAGCATCC | 116 |
| Anti-sens: AATTCAACTTCAGGATCCGC | ||
| mrp2 | Sens: GCTGGTTGGAAACTTGGTCG | 93 |
| Anti-sens: CAACTGCCACAATGTTGGTC | ||
| oatp2 | Sens: TGTGATGACCTGTGATAATTTTCCA | 81 |
| Anti-sens: TTCTCCACATATAGTTGGTGCTGAA | ||
| Gapdh | Sens: TAAAGGGCATCCTGGGCTACACT | 200 |
|
| Anti-sens: TTACTCCTTGGAGGCCATGTAGG |
|
Mdr1a, mrp2, and gapdh primers were designed based on published cDNA sequences with the help of the Jellyfish and BLASTIN 2.20.1 computer programs (Altschul et al., 1997). Oatp2 sequence was as previously published by Gao et al. (2004). The resulting PCR products were sequenced on an ABI Prism 3100 analyzer (Applied Biosystems, Foster City, CA)