TABLE 2

F2 values and -fold induction at highest concentration tested (given in parenthesis) for induction of CYP2B6 mRNA in HepaRG cells treated with rifampicin, omeprazole, troglitazone, phenytoin, primaquine, or phenobarbital F2 is the concentration resulting in a 2-fold increase of baseline mRNA levels. EC50 values are presented when achieved. Values are mean ± S.D. of three separate batches of differentiated HepaRG cells.




F2

-Fold Induction at Highest Concentration
Rifampicin 0.16 ± 0.11a 20 ± 13 (4 μM)
Omeprazole 2.3 ± 0.1 16 ± 8.7 (40 μM)
Troglitazone 1.9 ± 0.6 4.6 ± 2.7 (6.25 μM)
Phenytoin 1.7 ± 1.0 9.7 ± 5.6 (40 μM)
Primaquine N.I.b N.I.
Phenobarbital
11 ± 5.8
26 ± 15 (200 μM)
  • a μM

  • b N.I. = no induction recorded