TABLE 1

Ascorbate-dependent oxidation of SMX to SMX-OH by H2O2

Incubation
Incubation Componentsa
SMX-OH Generationb
1 SMX (800 μM) 0
2 1 + H2O2 (1 mM) 0
3 2 + ascorbate (1 mM) 0.262
4 3 + Fe2+ (100 mM) + EDTA (100 mM) 1.258
5 3 + mannitol (0.5 M) 0.283
6
3 + desferrioxamine (25 mM)
0.053

  • ↵ a SMX was incubated in 50 mM Tris buffer, pH 8.0, at 37°C for 1 h

  • ↵ b Formation of SMX-OH was assessed from the chromatographic peak-area ratio. SMX-OH: sulfadimethoxine at 254 nm