Representative purification of Met S-oxidase activity from rat kidney microsomes Met S-oxidase activities, protein concentrations, and purification steps were carried out as described under Materials and Methods. Incubation times were 20 min. Met concentration was 10 mM.
Fraction | Volume | Total Protein | Total Activity | Specific Activity | Purification |
|---|---|---|---|---|---|
| ml | mg | nmol/min | nmol/mg protein/min | -fold | |
| Microsomes | 17 | 122 | 117 | 0.96 | 1 |
| Solubilized microsomes | 35 | 56 | 100 | 1.8 | 1.9 |
| Blue pool I | 12.5 | 7.4 | 45 | 6.1 | 6.4 |
| Blue pool II | 6 | 4 | 43 | 10.8 | 11.3 |
| ADP pool | 1 | 0.04 | 5 | 125 | 130 |