Phenacetin dissociation constants (KD) Details of titration given in text and Fig. 1.
Protein | KD (μM) | [KD (mutant)/KD (2A13wt)] |
|---|---|---|
| 2A13* | 34 | 1.0 |
| L110V | 18 | 0.5 |
| A117V | 20 | 0.6 |
| S208I | 108 | 3.1 |
| A213S | 65 | 1.9 |
| F300I | N.A. | N.A. |
| A301G | N.A. | N.A. |
| M365V | 135 | 4.0 |
| L366I | 2.2 | 0.1 |
| G369S | 97 | 2.9 |
| H372R | 20. | 0.6 |
| 2A6 | N.A. | N.A. |
| I300F/G301A | 103 | 3.0 |
| I208S/I300G/G301A | 63 | 1.8 |
| I300F/G301A/G369S | 13 | 0.4 |
| I208S/I300F/G301A/G369S | 21 | 0.6 |
N.A., only a reduced spectral shift was detected, which did not allow determination of KD
↵* KD value represents the mean of three independent trials