Kinetic parameters of erythromycin N-demethylation by CYP3A4 Supersomes before and after CBZ-modification NADPH–, CBZ–: preincubation of CYP3A4 in the absence of NADPH and CBZ followed by assay of erythromycin N-demethylase; NADPH–, CBZ+: preincubation of CYP3A4 with CBZ but without NADPH followed by assay of erythromycin N-demethylase; NADPH+, CBZ+: preincubation of CYP3A4 in the presence of both NADPH and CBZ followed by assay of erythromycin N-demethylase.
CYP3A4 Supersomes | Vmax | Km |
|---|---|---|
| nmol/min/nmol | μM | |
| NADPH–, CBZ– | 3.27 ± 0.08 | 44.0 ± 4.0 |
| NADPH–, CBZ+ | 3.60 ± 0.58 | 80.0 ± 0.00 |
| NADPH+, CBZ+ | 3.61 ± 0.22 | 44.0 ± 4.0 |