UGT Isoform | pNP | UDP-GlcUA | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Vmax | Km | Vmax/Km | Vmax | Km | Vmax/Km | |||||
μM min–1 mg–1 protein | μM | min–1 mg–1 protein | μM min–1 mg–1 protein | μM | min–1 mg–1 protein | |||||
1A10 WT | 13.8 ± 0.6 | 323 ± 54 | 0.043 ± 0.007 | 7.0 ± 0.3 | 323 ± 54 | 0.022 ± 0.004 | ||||
D393A | ||||||||||
Q394A | ||||||||||
D396A | 4.7 ± 0.2 | 286 ± 56 | 0.016 ± 0.003 | 7.2 ± 0.6 | 490 ± 150 | 0.015 ± 0.004 | ||||
K399A | 67 ± 5 | 940 ± 170 | 0.071 ± 0.013 | 22 ± 2 | 480 ± 140 | 0.046 ± 0.013 |
WT, wild type