TABLE 2

Effects of clofibric acid on 2-PPA-CoA formation, acyl glucuronidation, and covalent binding in vivo in rat livers

Rats were pretreated i.p. with clofibric acid (160 mg/kg/day) or vehicle (0.1 M sodium bicarbonate) for 7 days. On day 8, rats received an i.p. injection of (RS)-[1-¹⁴C]2-PPA (130 mg/kg in 0.9% saline, 0.5 ml/rat). Livers were collected 2 h after (RS)-[1-¹⁴C]2-PPA administration. Values are expressed as the mean ± S.D. (n = 3).

	2 h After Administration of (RS)-[1-¹⁴C]2-PPA		AUC_{(0-2 h)} Ratio (Treated/Control)^a
	Control	Clofibric Acid-Treated	AUC_{(0-2 h)} Ratio (Treated/Control)^a
2-PPA-CoA (nmol/g liver)	73.6 ± 13.0	171 ± 7^b	2.9
2-PPA glucuronide (nmol/g liver)	589 ± 27	534 ± 57	0.79
Covalent binding (pmol/mg protein)	195 ± 16	243 ± 21^b

↵ a AUC_{(0-2 h)} in clofibric acid treated and control animals (n = 2 for each time points) was calculated from Fig. 2.
↵ b P < 0.05 versus control, using unpaired Student's t test.