Sequences of the primers used for site-directed mutagenesis
The codons to be mutated are underlined with the altered single nucleotides in bold.
| Variants | Direction | Sequences (5′→3′) |
|---|---|---|
| Arg48Gly | Forward | GGCGGCAGCTCGGGTCCGCGCCCC |
| Reverse | GGGGCGCGGACCCGAGCTGCCGCC | |
| Trp57Cys | Forward | CCCGTTTGCGTGCCCACTGATCGGAAAC |
| Reverse | GTTTCCGATCAGTGGGCACGCAAACGGG | |
| Gly61Glu | Forward | GGCCACTGATCGAAAACGCGGCGGCG |
| Reverse | CGCCGCCGCGTTTTCGATCAGTGGCC | |
| Ala119Ser | Forward | CCGACCGGCCGTCCTTCGCCTCCT |
| Reverse | AGGAGGCGAAGGACGGCCGGTCGG | |
| Gly365Trp | Forward | GGATCAGGTCGTGTGGAGGGACCGTCTG |
| Reverse | CAGACGGTCCCTCCACACGACCTGATCC | |
| Pro379Leu | Forward | CAGCCCAACCTGCTCTATGTCCTGGCCT |
| Reverse | AGGCCAGGACATAGAGCAGGTTGGGCTG | |
| Glu387Lys | Forward | CCTGGCCTTCCTTTATAAAGCCATGCGCTTCTCC |
| Reverse | GGAGAAGCGCATGGCTTTATAAAGGAAGGCCAGG | |
| Arg390His | Forward | CTTTATGAAGCCATGCACTTCTCCAGCTTTGTGC |
| Reverse | GCACAAAGCTGGAGAAGTGCATGGCTTCATAAAG-3′ | |
| Leu432Val | Forward | GTGAATCATGACCCAGTGAAGTGGCCTAAC |
| Reverse | GTTAGGCCACTTCACTGGGTCATGATTCAC | |
| Pro437Leu | Forward | GTGAAGTGGCCTAACCTGGAGAACTTTGATCCAG |
| Reverse | CTGGATCAAAGTTCTCCAGGTTAGGCCACTTCAC | |
| Ala443Gly | Forward | GAGAACTTTGATCCAGGTCGATTCTTGGACAAGG |
| Reverse | CCTTGTCCAAGAATCGACCTGGATCAAAGTTCTC | |
| Asn453Ser | Forward | GGATGGCCTCATCAGCAAGGACCTGACCAG |
| Reverse | CTGGTCAGGTCCTTGCTGATGAGGCCATCC | |
| Arg469Trp | Forward | CAGTGGGCAAAAGGTGGTGCATTGGCGAAG |
| Reverse | CTTCGCCAATGCACCACCTTTTGCCCACTG |