Genes analyzed by single-gene real-time PCR, their respective Taqman Gene Expression Assay numbers, and GenBank Accession numbers
TaqMan primers and probe were synthesized by Applied Biosystems (Foster City, CA).
| Species | Gene Name | Gene Description | Taqman Assay No. | GenBank Accession No. |
|---|---|---|---|---|
| Rat | HNF1α | Transcription factor 1, Tcf1 | Rn00562020_m1 | NM_012669.1 |
| Rat | PPARα | Peroxisome proliferator-activated receptor α | Rn00566193_m1 | NM_013196.1 |
| Rat | AhR | Aryl hydrocarbon receptor | Rn00565750_m1 | NM_013149.2 |
| Rat | Cyp2B1 | Cytochrome P450, family 2, subfamily b, polypeptide 1 | Custom* | AJ_320166.1, U30327.1 |
| Rat | Cyp3A1 | Cytochrome P450, family 3, subfamily a, polypeptide 23/polypeptide 1 | Rn01640761_gH | NM_013105.2 |
| Rat | MDR1 | ATP-binding cassette, subfamily B (MDR/TAP), member 1B | Rn00561753_m1 | NM_012623.2 |
| Human | HNF1α | Transcription factor 1 (TCF1), hepatic nuclear factor (HNF1) | Hs00167041_m1 | NM_000545.4 |
| Human | PPARα | Peroxisome proliferator-activated receptor α | Hs00231882_m1 | NM_001001928.2, NM_005036.4 |
| Human | AhR | Aryl hydrocarbon receptor | Hs00169233_m1 | NM_001621.3 |
| Human | CAR | Nuclear receptor subfamily 1, group I, member 3, NR1I3 | Hs00231959_m1 | NM_005122.2 |
| Human | CYP4A11 | Cytochrome P450, family 4, subfamily A, polypeptide 11 | Hs00167961_m1 | NM_000778.2 |
| Human | MDR1 | ATP-binding cassette, subfamily B (MDR/TAP), member 1 | Hs00184500_m1 | NM_000927.3 |
HNF, hepatic nuclear factor.
↵* Sequences (all 5′ → 3′) TaqMan probe CCCACAGACAAATCT, forward primer GAGTTCTTCTCTGGGTTCCTGAAAT, reverse primer ACAATATGGCCAATGTAATCGAGGAT.