TABLE 1

PCR and sequencing primers and cycle conditions used for BCRP resequencing and for real-time quantification

Region Amplified Relative to TSS Position Primer Sequence (5′→3′) Amplicon Size Annealing/Extension Conditions
bp °C
AC097484
    Region I -71599--72533 137616F AAGGAGAAGCCCAACATCCT 934 59/72
138550R CCTTCTTCTAGCCCCTTGCT
    Region II -28916--30930 94933F AATGGGGCAGTTTCTTACCA 2014 59/68
96947R TGGTGCCATCAAACTGAAAG
    Sequence 95508R TAGAGATGAGGTCTCACTGTGTTG
96237F TCTTGCAGCTCCTTGGTTAAA
96257R TTTAACCAAGGAGCTGCAAGA
AC084732
    Region III -18132--14320 2227F TTTGCTTCTCTTTTCCATAGCTC 3812 59/68
6039R AGTGGCCAATCTTTGTTTCA
    Sequence 3556R GTAGTCTCCGCGTGCATTCT
4090F GACGCAATCATGTAGAACATAACA
5273R ATGGACAGCAGTGTGTCCTTGAGAAATT
2792F AAAAAGCATTCTCTTTGGTTTCA
    Region IV -13890--13400 6469F AAAAACCCCTCCTGCAAAAT 490 59/72
6959R GCCTAGTGGCTCATGCCTAT
    Region V -13601--11457 6758F AACCTCTGCCTCCTTGGTTC 2144 59/72
8902R GGGCCTAATCCTGACACAAA
    Sequence 7475R TTGAGGGTGGAGGTTGAGAG
8055F CGTGTCATGAGGGTTTGTTG
    Region VI -6594--4577 13765F GGGAGGCAGAAGAAAATCAA 2017 58/72
15782R TGAAATGGGATCCAGAGGAA
    Sequence 14273F AAGACCAGCTGAGGCAACAT
    Region VII -4203--1369 16156F CGGACTGAATACATCTGTTATAT 2834 57/72, + MgCl2
18990R GGCCGTTAACGACTGTTTGCAA
    Sequence 16900R AAAGACACATGACAATAGAGTCCTCA
17018F GACATTCCATGTTACTACCCAGAA
    Region VIII -814-+1621 19545F TGCATTGGGTGAACCATTAATA 2435 59/68 +7.5% dimethyl sulfoxide
21980R CAATGAAAGGCTGAGGAACTG
    Sequence 21332R TTTAGCAAACCATCCAAAGC
19937F AAATGGGTGGTTTCTGGTGA
21308F AAATTATTGGTCAATCCCTTTAAAG
    Region IX 6711-7596 27070F TCAAATGAAACCCTGCCAGT 885 59/72
27955R TTACCCAAAACCACACAGCA
    Region X 9296-10427 29655F CAAAGTGCTGGCATTACAGG 1131 59/72
30786R TGATCAGGAGCTAAGACTTGACC
    Sequence 30583R GGAAGGGATCCTATGCCAAC
    Region XI 11481-13553 31840F GGCAAAGAGCATGAAGAAG 2072 60/72
33912R GTTGATCCATGGGCTTTCAT
    Sequence 32409F TCTGTTGCTTAGGCTGGATTC
    Region XII 13745-17404 34104F TTTATGCAGCATGCCCTATG 3659 59/68
37763R GAGGAGCCCTGGAAAGAGAT
    Sequence 34500R TGAGAAGTTTAACCATTTGTCTTC
35645F TCCTATCACAGGAAAGGCAAA
36776F TTGAATTTGCTGGGTGTCAG
    Intron 2f 44341F GGAAAGCTTTTCTGACAGTGG 2462 60/72
    Intron 2r 46803R GCGTTGCAAATGCTCAATAA
    Sequence 45246R AGACTTCAGGGTGGGGTCTG
45750R CGTGGTACATACATAAGCACGTT
    Intron 7F 61087F CACCTTATTGGCCTCAGGAA 1596 60/72
    Intron 7R 62683R AATCAACTGCTGTGCTGCTG
    Sequence 61756R GCAGTCCCAGCTACTCAGGA
    Intron 10f 69358F GTGCCAAGGGTTTTCTGAAC 4221 63/72, +MgCl2
    Intron 10r 73579R GGAGCACAGTATCTGCCACA
    Sequence 70728F CCAGCCCATTTCTTGTTTTT
71488R GGGGAGAGTGGTAGGACACA
71476F TACCACTCTCCCCAAAGCAC
72283R TGGATGGTGGTGATAGTTGC
    Intron 14f 82983F CAATGCCAGGTGTATTGGTG 3533 60/72
    Intron 14r 86516R AAAGGAGCCTAAAATTGAACCA
    Sequence 83688R AGGAGGTATCTCCCCTAGCC
83665F AAAGGGCTAGGGGAGATACC
84434R CAAAACCCATTTTGACACTGAA
84612F CATGCAGCAATGTTTCTTACG
85419R AGCCTCATTTCCGTTACCA
Reverse transcription-PCR primers
AC084732
BCRP exon 1b-f 20049F AACCCAGCTAGGTCAGACGA
NM-004827
BCRP exon 1a-f 437F CCTGAGCCTTTGGTTAAGACC
BCRP exon 6R 1057R
BCRP real-time f1 1304F CAGGAGGCCTTGGGATACTT
BCRP real-time f2 1324F TGAATCAGCTGGTTATCACTG
BCRP real-time r 1411R TGCCACAGCAGTGGAATCT
BCRP exon 8F 1348F GGCCTATAATAACCCTGCAGAC
BCRP exon 10R 1739R GCCCAAAGTAAATGGCACCT
BCRP exon 14F 2152F TCTGTTGGTCAATCTCACAACC
BCRP exon 16R 2431R CAGGTAGGCAATTGTGAGGAA
BC092408
BCRP exon 1c-f 70F CTCGGAAAGCCTCAATGTTC
Copy number primers
CYP3A4 CNVF CCCAGACTTGGCCATGGAAACC
CYP3A4 CNVR CAGATAAGGGAAAGAGAGGC

  • TSS, transcription start site.