TABLE 3

Comparison of transporter gene expression between Fa2N-4 cells and cryopreserved human hepatocytes

The gene expression levels were evaluated in human hepatocytes and Fa2N-4 cells after the 24- and 48-h adaptation periods, respectively. Data are expressed as means ± S.E.-fold change in human hepatocytes compared with Fa2N-4 cells determined from three independent experiments, as described under Materials and Methods.

Pubmed ID
Gene
Basal Expression Levels Relative to Fa2N-4
527
455
LHO
DMQ
NM_000927 ABCB1a,b 0.46 ± 0.09* 0.26 ± 0.05* 0.42 ± 0.12* 0.62 ± 0.10*
NM_003742 ABCB11 >100c,* >100c,* 93.3d >100c,*
NM_000443 ABCB4 0.34 ± 0.10* 1.13 ± 0.31 1.64 ± 1.27 1.64 ± 0.74
NM_004996 ABCC1 0.13 ± 0.05* 0.03 ± 0.00* 0.10 ± 0.04* 0.05 ± 0.03*
NM_000392 ABCC2 2.26 ± 0.60* 1.72 ± 0.45* 5.33 ± 0.66* 5.26 ± 0.42*
NM_003786 ABCC3 a 1.11 ± 0.13 0.40 ± 0.04* 0.79 ± 0.07* 2.44 ± 0.09*
NM_005845 ABCC4 0.81 ± 0.31 0.61 ± 0.25 0.49 ± 0.11* 0.63 ± 0.11
NM_001171 ABCC6 a 0.89 ± 0.01* 0.33 ± 0.11* 0.45 ± 0.02* 1.98 ± 0.37
NM_004827 ABCG2 a 0.50 ± 0.01* 0.22 ± 0.08* 0.89 ± 0.35 0.56 ± 0.08
NM_003049 SLC10A1 >100c,* >100c,* >100c,* >100c,*
NM_003057 SLC22A1 16.01 ± 1.95* 7.89 ± 3.36* 27.43 ± 6.77* >100c,*
NM_153320 SLC22A7 1.83 ± 0.62* 2.08 ± 0.56* 1.03 ± 0.49 16.33 ± 2.47*
NM_134431 SLCO1A2 1.04 ± 0.83 5.82 ± 1.01* 34.87 ± 24.52 22.88 ± 19.73
NM_006446 SLCO1B1 b >100c,* >100c,* >100c,* >100c,*
NM_019844 SLCO1B3 55.09 ± 22.10* >100c,* >100c,* >100c,*
NM_007256
SLCO2B1
2.83 ± 1.02*
1.10 ± 0.30
2.59 ± 0.48*
12.89 ± 2.65*

  • ↵* P < 0.05, ΔCt value significantly different from that for Fa2N-4 cells.

  • ↵ a Fa2N-4 cells gene expression increased >10-fold during the 48-h adaptation period.

  • ↵ b Cryopreserved human hepatocyte gene expression increased >10-fold during the 24-h adaptation period.

  • ↵ c Low gene expression in Fa2N4 cells (Ct >34).

  • ↵ d Data are expressed as the average value of experiments performed in duplicate.