Gene expression analysis of different ATF factors from oligonucleotide array data
Total RNA was isolated from different human liver samples (n = 6), cultured human hepatocytes (48-h culture) (n = 3), and human hepatoma HepG2 cells (70% confluence) (n = 3). RNA integrity was assessed by using an Agilent Bioanalyzer (Agilent Technologies, Palo Alto, CA). cRNAs were prepared according to one-cycle target-labeling protocol starting from 5 μg of total RNA and hybridized to HG-U133 GeneChip oligonucleotide arrays (Affymetrix, Santa Clara, CA). After image analysis and array normalization, intensity data were expressed as -fold increase relative to liver tissue.
ATF5 | ATF5 | ATF4 | ATF3 | ATF2 | ATF2 | ||
|---|---|---|---|---|---|---|---|
| [204998_s_at] | [204999_s_at] | [200779_at] | [202672_s_at] | [205446_s_at] | [212984_at] | ||
| Liver tissue | Mean | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| S.D. | 0.19 | 0.25 | 0.34 | 0.36 | 0.28 | 0.20 | |
| Hepatocytes | Mean | 0.31 | 0.20 | 1.73 | 1.60 | 1.68 | 1.02 |
| S.D. | 0.06 | 0.06 | 0.87 | 1.22 | 0.51 | 0.42 | |
| HepG2 | Mean | 0.02 | 0.02 | 0.98 | 0.89 | 3.25 | 1.29 |
|
| S.D. | 0.00 | 0.00 | 0.12 | 0.18 | 0.18 | 0.35 |