Gene expression in HepaRG cells as a function of time at confluence
Comparative expression of various genes in differentiated HepaRG cells at several confluent time points. Results are expressed as percentages compared with day 30 after seeding, arbitrary set at 100%, and are the mean ± S.E.M. of three independent experiments. In parentheses are the percentages of the values measured in a pool of freshly isolated human hepatocytes from three donors.
Genes | mRNA Levels (%) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 30 | 37 | 44 | 51 | 58 | p | ||||||
| days | |||||||||||
| Aldolase B | 100 (6) | 113 ± 14 | 129 ± 38 | 155 ± 36 | 154 ± 28 | N.S. | |||||
| CYP3A4 | 100 (119) | 99 ± 11 | 108 ± 47 | 111 ± 31 | 105 ± 15 | N.S. | |||||
| CYP1A2 | 100 (1) | 111 ± 35 | 128 ± 25 | 109 ± 42 | 93 ± 38 | N.S. | |||||
| CYP2B6 | 100 (32) | 117 ± 12 | 185 ± 9 | 181 ± 24 | 208 ± 71 | N.S. | |||||
| CYP2E1 | 100 (5) | 207 ± 38 | 187 ± 13 | 169 ± 38 | 133 ± 16 | N.S. | |||||
| GSTA1/2 | 100 (186) | 90 ± 15 | 89 ± 18 | 106 ± 15 | 94 ± 10 | N.S. | |||||
| UGT1A1 | 100 (181) | 81 ± 8 | 96 ± 11 | 90 ± 3 | 81 ± 19 | N.S. | |||||
| mEH | 100 (213) | 101 ± 10 | 105 ± 3 | 111 ± 13 | 114 ± 16 | N.S. | |||||
| BSEP | 100 (2) | 93 ± 36 | 150 ± 84 | 181 ± 55 | 233 ± 37 | N.S. | |||||
| MDR1 | 100 (303) | 81 ± 10 | 89 ± 3 | 71 ± 9 | 72 ± 12 | N.S. | |||||
| OCT1 | 100 (25) | 124 ± 25 | 159 ± 20 | 181 ± 12 | 173 ± 26 | N.S. | |||||
| MRP2 | 100 (164) | 112 ± 9 | 133 ± 4 | 116 ± 11 | 117 ± 22 | N.S. | |||||
| CAR | 100 (21) | 111 ± 4 | 143 ± 47 | 143 ± 26 | 136 ± 14 | N.S. | |||||
| PXR | 100 (58) | 132 ± 23 | 172 ± 32 | 176 ± 28 | 188 ± 59 | N.S. | |||||
| MnSOD | 100 (317) | 105 ± 17 | 145 ± 10 | 144 ± 22 | 121 ± 31 | N.S. | |||||
| Catalase | 100 (67) | 84 ± 2 | 100 ± 20 | 88 ± 16 | 89 ± 18 | N.S. | |||||
N.S., not statistically significant.