Analytical conditions in LC/MS/(MS) analysis
Compound | Ionization Mode | Detection Mode | m/z | Mobile Phase | Gradient Condition (B Concentration %) | ||
|---|---|---|---|---|---|---|---|
| A | B | ||||||
| MeIQx | ESI, positive | SIM | 214 | 10 mM Acetic ammonium | Acetonitrile | 0 min; 5% → 1.5 min; 50% → 3 min; 50% → 3.01 min; 5% → 6 min; 5% | |
| PhIP | APCI, positive | MRM | 225/210 | 10 mM Acetic ammonium | Acetonitrile | 0 min; 10% → 0.2 min; 10% → 2.2 min; 60% → 3 min; 70% → 3.01 min; 10% → 4 min; 10% | |
| 4′-OH PhIP | APCI, positive | MRM | 241/226 | 10 mM Acetic ammonium | Acetonitrile | 0 min; 10% → 0.2 min; 10% → 2.2 min; 60% → 3 min; 70% → 3.01 min; 10% → 4 min; 10% | |
| N-OH PhIP | APCI, positive | MRM | 241/223 | 10 mM Acetic ammonium | Acetonitrile | 0 min; 10% → 0.2 min; 10% → 2.2 min; 60% → 3 min; 70% → 3.01 min; 10% → 4 min; 10% | |
| Dantrolene | ESI, negative | SIM | 313 | 0.05% Formic acid | Acetonitrile | 0 min; 25% → 1.5 min; 90% → 2.5 min; 90% → 2.51 min; 25% → 5 min; 25% | |
| Prazosin | ESI, positive | SIM | 384 | 10 mM Acetic ammonium | Acetonitrile | 0 min; 20% → 1.5 min; 80% → 2.5 min; 80% → 2.51 min; 20% → 5 min; 20% | |
| Triamterene | ESI, positive | SIM | 254 | 0.05% Formic acid | Acetonitrile | 0 min; 8% → 1.5 min; 20% → 2.5 min; 20% → 2.51 min; 8% → 5 min; 8% | |
APCI, atmospheric pressure chemical ionization; ESI, electrospray ionization; MRM, multiple reaction monitoring; SIM, selected ion monitoring.