TABLE 1

Details of analytical methods


Assay

Mobile Phase

Gradient Profile

Detection Method

Retention Times
Curcuminoids 10 mM ammonium acetate (pH 4.2) 40% CH3CN (0–20 min) → 75% CH3CN (25 min) → 40% CH3CN (26–28 min) Curcuminoids: 425 nm; Phenacetin: 254 nm Curcumin: 25.2 min; Demethoxy: 24.6 min; Bisdemethoxy: 23.6 min; Phenacetin (IS): 3.6 min
Triazolam 10 mM phosphate buffer (pH 7.0) 25% CH3CN/ 15% CH3OH (0–17 min) → 32% CH3CN/ 20% CH3OH (18 min) → 45% CH3CN/ 20% CH3OH (25 min) → 25% CH3CN/ 15% CH3OH (27–32 min) 220 nm 1′-OH-triazolam: 15.2 min; 4-OH-triazolam:16.7 min; Diazepam (IS): 26.5 min
Flurbiprofen 20 mM phosphate buffer (pH 2.2) 35% CH3CN (0 min) → 50% CH3CN (5 min) → 75% CH3CN (15 min) → 35% CH3CN (17–20 min) 4′-OH-flurbiprofen: ex 260 nm, em 320 nm; Phenacetin: 220 nm 4′-OH-flurbiprofen: 8.9 min; Phenacetin (IS): 6.5 min
S-Mephenytoin 0.5% formic acid 10% CH3OH (0 min) → 80% CH3OH (4 min) → 10% CH3OH (4.1–12 min) 4-OH-mephenytoin (MS-MS): 235.5 (width 5) → 150.2 4-OH-mephenytoin: 3.7 min
Diazepam (MS): 285.3 Diazepam (IS): 5.4 min
Dextromethorphan 20 mM phosphate buffer (pH 2.2) 20% CH3CN (0–5 min) → 60% CH3CN (14 min) → 20% CH3CN (15–18 min) ex 280 nm, em 310 nm Dextrorphan: 7.8 min; Pronethalol (IS): 12.1 min
Phenacetin 0.5% formic acid 0% CH3CN (0 min) → 50% CH3CN (8 min) → 0% CH3CN (8.1–12 min) APAP (+, MS-MS): 152.1 (1.0 width) → 110.0 APAP: 2.9 min; Sulfaphenazole (IS): 6.6 min
Sulfaphenazole (+): 315.3
Chlorzoxazone 20 mM phosphate buffer (pH 2.2) 18% CH3CN (0 min) → 50% CH3CN (15 min) → 18% CH3CN (16–20 min) 295 nm 6-OH-chlorzoxazone: 8.8 min
Phenacetin (IS): 12.8 min
Bupropion 0.02% TFA 5% CH3CN (0 min) → 60% CH3CN (5 min) → 5% CH3CN (5.2–10 min) Hydroxybupropion (+, MS-MS): 256.0 (2.0 width) → 238.0 Hydroxybupropion: 2.1 min
Trazodone (+): 372.6 Trazodone (IS): 4.1 min
Acetaminophen (+ Mode) 10 mM ammonium acetate (pH 4.2) 0% CH3CN (0–4 min) → 50% CH3CN (14 min) → 0% CH3CN (14.1–23 min) APAP-glucuronide (MS-MS): 345.2 (10 width) → 152.2 APAP-glucuronide: 1.4 min Sulfaphenazole (IS): 10.7 min
Sulfaphenazole: 315.3
Acetaminophen (– Mode) 10 mM ammonium acetate (pH 4.2) 0% CH3CN (0 min) → 50% CH3CN (10 min) → 0% (10.1–15 min) APAP-sulfate (MS-MS): 230.2 (10 width) → 150.2 APAP-sulfate: 2.9 min



Sulfaphenazole: 313.3
Sulfaphenazole (IS): 7.1 min
  • IS, internal standard; ex, excitation; em, emission