TABLE 2

[14C]C6-ceramide and [3H]DSPC tissue biodistribution Tissue distribution data are expressed as percentages of injected dose per organ at different time points for [3H]DSPC ceramide liposomes, [3H]DSPC control liposome, and [14C]C6-ceramide. Data are means ± S.D. from n = 4- to 5 rats.

Tissue
2 min
1 h
2 h
48 h
% injected dose/organ
[3H]DSPC ceramide liposomes
   Plasma 89.40 ± 5.07 68.24 ± 17.29 56.52 ± 3.00 5.97 ± 1.53
   Spleen 0.32 ± 0.13 1.63 ± 0.31 1.55 ± 0.44 1.53 ± 0.20
   Liver 10.32 ± 0.85 19.49 ± 8.79 16.91 ± 1.17 10.71 ± 1.14
   Lung 2.94 ± 1.31 1.30 ± 0.15 1.27 ± 0.34 0.66 ± 0.09
   Heart 0.68 ± 0.18 0.62 ± 0.10 0.57 ± 0.03 0.32 ± 0.02
   Kidney 1.20 ± 0.20 1.20 ± 0.11 1.46 ± 0.22 0.80 ± 0.04
   Brain 0.30 ± 0.04 0.28 ± 0.04 0.31 ± 0.11 0.32 ± 0.04
   Total 105.17 ± 3.67 92.76 ± 16.55 78.58 ± 3.38 20.30 ± 2.33
[3H]DSPC control liposomes
   Plasma 52.48 ± 5.73 5.83 ± 2.10
   Spleen 1.12 ± 0.18 0.91 ± 0.16
   Liver 16.88 ± 1.88 10.03 ± 0.65
   Lung 1.64 ± 0.25 0.68 ± 0.15
   Heart 0.64 ± 0.08 0.33 ± 0.03
   Kidney 1.20 ± 0.21 0.87 ± 0.07
   Brain 0.31 ± 0.03 0.35 ± 0.10
   Total 74.26 ± 5.52 19.00 ± 1.98
[14C]C6-ceramide
   Plasma 15.14 ± 3.79 3.54 ± 0.47 1.70 ± 0.15 0.166 ± 0.019
   Spleen 0.89 ± 0.39 0.16 ± 0.01 0.09 ± 0.03 0.027 ± 0.003
   Liver 22.80 ± 1.75 5.68 ± 0.84 3.88 ± 0.56 0.390 ± 0.041
   Lung 8.48 ± 0.37 0.65 ± 0.13 0.29 ± 0.03 0.035 ± 0.004
   Heart 1.47 ± 0.44 0.35 ± 0.02 0.11 ± 0.01 0.013 ± 0.001
   Kidney 1.64 ± 0.17 1.22 ± 0.25 0.63 ± 0.07 0.048 ± 0.003
   Brain 0.34 ± 0.06 0.16 ± 0.02 0.11 ± 0.01 0.036 ± 0.003
   Total
50.75 ± 2.08
11.76 ± 1.11
6.82 ± 0.49
0.715 ± 0.059