[14C]C6-ceramide and 3H-DSPC tissue biodistribution Tissue distribution data are expressed as percentages of injected dose per gram of tissue at different time points for [3H]DSPC ceramide liposomes and [14C]C6-ceramide. Data are means ± S.D. from n = 4 to 5 rats.
Tissue | 2 min | 1 h | 2 h | 48 h | |
|---|---|---|---|---|---|
| % injected dose/g | |||||
| [3H]DSPC ceramide liposomes | |||||
| Plasma | 8.94 ± 0.51 | 6.82 ± 1.73 | 5.65 ± 0.30 | 0.60 ± 0.15 | |
| Spleen | 0.61 ± 0.19 | 3.67 ± 0.43 | 4.23 ± 1.12 | 3.13 ± 0.44 | |
| Liver | 1.16 ± 0.18 | 2.21 ± 0.82 | 1.82 ± 0.24 | 1.13 ± 0.10 | |
| Lung | 1.89 ± 0.40 | 1.33 ± 0.22 | 1.26 ± 0.36 | 0.52 ± 0.05 | |
| Heart | 0.86 ± 0.17 | 0.79 ± 0.06 | 0.72 ± 0.10 | 0.40 ± 0.01 | |
| Kidney | 0.73 ± 0.14 | 0.61 ± 0.07 | 0.74 ± 0.15 | 0.43 ± 0.02 | |
| Brain | 0.17 ± 0.02 | 0.15 ± 0.02 | 0.17 ± 0.06 | 0.17 ± 0.02 | |
| [14C]C6-ceramide | |||||
| Plasma | 1.51 ± 0.38 | 0.35 ± 0.05 | 0.17 ± 0.02 | 0.017 ± 0.002 | |
| Spleen | 1.66 ± 0.59 | 0.37 ± 0.05 | 0.26 ± 0.06 | 0.055 ± 0.003 | |
| Liver | 2.56 ± 0.33 | 0.66 ± 0.05 | 0.42 ± 0.05 | 0.041 ± 0.004 | |
| Lung | 5.74 ± 0.80 | 0.66 ± 0.11 | 0.29 ± 0.04 | 0.028 ± 0.002 | |
| Heart | 1.85 ± 0.39 | 0.44 ± 0.03 | 0.14 ± 0.02 | 0.017 ± 0.001 | |
| Kidney | 0.99 ± 0.12 | 0.62 ± 0.13 | 0.32 ± 0.04 | 0.026 ± 0.001 | |
| Brain | 0.19 ± 0.03 | 0.09 ± 0.01 | 0.06 ± 0.01 | 0.019 ± 0.001 | |