Primer sequences and thermal cycling conditions used for analysis of steady-state mRNA levels in rat liver by RT-PCR
Target | Forward and Reverse PCR Primer Sequences | PCR Product Size | Thermal Cycling Parameters (Denature; Anneal; Extend) × cycles | Reference |
|---|---|---|---|---|
| bp | ||||
| CYP1A1 | 5′- 627-ACGTTATGACCACGATGACC-646-3′ (FP) | 672 | (20 s/94°; 20 s/52°; 40 s/72°) × 17 | Franc et al., 2001 |
| 5′- 1299-AGGCCGGAACTCGTTTG-1283-3′ (RP) | ||||
| β-Actin | 5′-344-ACCGTGAAAAGATGACCCAG-363-3′ (FP) | 688 | (20 s/94°; 20 s/52°; 40 s/72°) × 17 | Franc et al., 2001 |
|
| 5′-1031-GAGCCACCAATCCACACAG-1011-3′ (RP) |
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bp, base pairs; FP, forward primer; RP, reverse primer.