Sequence of the two potential CMPK translation initiation codons and results of studies performed to determine which codon might be functional in vivo
See also Fig. 2.
ATG1 | ATG2 | COS-1 Cell Western Blot Results | RRL Results | |
|---|---|---|---|---|
| Ideal Kozak sequence | ACCATGG | ACCATGG | ||
| WT ATG1/WT ATG2 | TGTATGC | CTCATGA | 196 amino acids | 196 amino acids |
| Mut ATG1/WT ATG2 | TGTTTGC | CTCATGA | 196 amino acids | 196 amino acids |
| Kozak ATG1/WT ATG2 | ACCATGG | CTCATGA | 228 and 196 amino acids | 228 and 196 amino acids |
| Kozak ATG1/Mut ATG2 | ACCATGG | CTCTTGA | 228 amino acids | |
| WT ATG1/Mut ATG2 | TGTATGC | CTCTTGA | No protein | No protein |
RRL, rabbit reticulocyte lysate.