Recombinant DCK and CMPK allozyme substrate kinetics
Gemcitabine substrate kinetics for WT and variant DCK allozymes and gemcitabine monophosphate substrate kinetics for CMPK were measured in the presence of 1 mM ATP. Values represent mean ± S.E.M. (n ≥ 6).
Apparent Km | |
|---|---|
| μM | |
| DCK allozyme | |
| WT | 14.6 ± 3.5 |
| Val24 | 13.1 ± 3.2 |
| Gly119 | 14.5 ± 2.5 |
| Ser122 | 14.1 ± 3.3 |
| Val24/Gly119/Ser122 | 14.8 ± 2.5 |
| Gly119/Ser122 | 9.5 ± 2.5 |
| Val24/Gly119 | 10.7 ± 1.1 |
| Val24/Ser122 | 6.3 ± 0.8* |
| CMPK allozyme | |
| WT | 246 ± 61 |
| His48 | 208 ± 45 |
| Lys75 | 265 ± 67 |
| Ser83 | 236 ± 39 |
↵* Statistically significant difference from the WT allozyme (P < 0.025 by Student's t test).