Enzyme | Benzydamine N-Oxidase Activity | Sulindac S-Oxidase Activity | ||
---|---|---|---|---|
Km | Vmax | Km | Vmax | |
μM (±S.E.) | nmol/min/nmol* (±S.E.) | μM (±S.E.) | nmol/min/nmol* (±S.E.) | |
dFMO3 | 18.6 (±4.5) | 0.63 (±0.03) | n.d. | >6.8 |
hFMO3 | 42.6 (±7.7) | 3.56 (±0.16) | 69.3 (±8.7) | 35.4 (±1.7) |
n.d., the Km value for dFMO3 could not be determined, because the enzyme did not display saturability at concentrations through 1000 μM of sulindac sulfide.
↵* Activity levels were normalized to nmol of catalytically active enzyme (FAD content) used in the assay, as determined by liquid chromatography-fluorescence.