Immunostaining conditions for detection and quantification of sulfotransferase isoforms
Incubation time for all the antibodies was 1 h.
| SULT1A1/1A3 | SULT1B1 | SULT2A1 | SULT1E1 | |
|---|---|---|---|---|
| 1° Antibody | ||||
| Antigen | Recombinant human SULT1A3 | Multiple antigenic peptide (MLSPKDILRKDLKLVHG) | Recombinant human SULT2A1 | Commercial antibody (N-terminal peptide) |
| Host | Sheep | Sheep | Sheep | Rabbit |
| Purification | IgG | IgG/peptide affinity | IgG | None |
| Dilution | 1:30,000 | 1:1000 | 1:50,000 | 1:8000 |
| 2° Antibody | ||||
| Dilution | 1:5000 | 1:5000 | 1:5000 | 1:5000 |